Dr. Coleman’s research focuses on understanding the processes involved in genome instability following genotoxic stresses such as ionizing radiation. Dr. Coleman’s research also uses novel approaches in nanotechnology using nanolipoproteins to help characterize biological components involved in cell signaling. Dr. Coleman’s training is in molecular biology where he received his B.A. from the University of Massachusetts, and his Ph.D. in biophysical studies of membrane proteins from Boston University. He has authored over 60 publications in peer-reviewed journals, published proceedings and book chapters covering a diverse breadth of molecular biology and biochemistry. Dr. Coleman has three patents and 6 patents pending related to biomarker discovery and biotechnology. For his work in the area of nanolipoproteins -membrane protein complexes Dr. Coleman received a Nanotechnology 50 award for co-development of his innovative approach to produce and solubilize membrane proteins using nanoparticles. Research projects in Dr. Coleman’s laboratory include radiation biology and applications to point-of-care detection using biosensors: - Biodosimetry - Human genetic variation - Cancer detection - Adaptive responses - Biological responses to radiotherapy His research work has been supported by several agencies including DOE, NIH and LLNL. Annotated Publications 1. Martinez, A., Hinz, J., Gomez, L., Molina, B., Acuna, H., Frias, S. and Coleman, M.A. (2008). Differential expression of TP53 associated genes in fanconi anemia cells after mitomycin C and hydroxyurea treatment. Mutat. Res. 656, 1-7. 2. Cappuccio, J.A., Blanchette, C.D., Sulchek, T., Arroyo, E. S. Hinz, A.K. Chromy, B.A. Fletcher, J., Katzen, F., Kudlicki, W., Bench, G., Hoeprich, P.D. and Coleman, M.A. (2008) Self-assembly of solubilized functional integral-membrane proteins in nanolipoprotein complexes using cell-free co-expression. Mol. Cell. Proteomics. 7, 2246-2253. 3. Katzen F. Fletcher, J., Kang, D., Peterson, T., Cappuccio, J.A., Blanchette, C.D., Shulchek, T., Chromy, B., Hoeprich, P., Coleman, M.A. and Kudlicki, W. (2008) Cell-free expression of integral membrane proteins into discoidal membranes. J. Proteome Res. 7:3535-3542. 4. Narayan, S., Lehmann, J., Coleman, M.A., Vaughan, A., Yang, C.C., Enepekides, D., Farwell, G., Purdy, J.A., Laredo, G. Nolan, K., Pearson, F. and Vijayakumar, S. (2008). Prospective evaluation to establish a dose response for clinical oral mucositis in patients undergoing head and neck conformal radiotherapy. Int J Radiat Oncol Biol Phys. 72:756-62. 5. Peterson, L.E. and Coleman, M.A. (2008) Machine learning-based receiver operating characteristic (ROC) curves for crisp and fuzzy classification of DNA microarrays in cancer research. Int J Approx Reason. 47:17-36. 6. Beller, H. R., Legler, T.C., Bourguet, F., Letain, T. E., Kane, S.R., and Coleman, M.A. (2009). Identification of c-type cytochromes involved in anaerobic, bacterial U(IV) oxidation. Biodegradation. 20:45-53. 7. Blanchette, C.D., Law, R.W., Benner, H., Pesavento, B., Cappuccio, J.A., Walsworth, V., Kuhn, E., Corzett, M., Chromy, B., Segelke, S., Coleman, M.A., Bench, G., Hoeprich, P., and Sulchek, T. (2009) Quantifying size distributions of nanolipoprotein particles (NLPs): A combined atomic force microscopy (AFM), ion mobility spectrometry (IMS) and molecular dynamic (MD) simulation study. J Lipid Res. 49:1420 8. Blanchette, C.D., Cappuccio, J.A., Kuhn, E.A., Segelke, B.W., Coleman, M.A., Chromy, B.A., Bench, G., Hoeprich, P.D., Sulchek, T.A. (2009) Atomic Force Microscopy Differentiates Discrete Size Distributions between Membrane Protein Containing and Empty Nanolipoprotein Particles. Biochimica et Biophysica Acta - Biomembranes. 1788:724-31. 9. Thompson, D., Pearson, F., Rao, R., Matthews, D. Albala, A., Wachsmann-Hogiu, S., and Coleman, M.A. (2009) A portable hand-held microarray reader for biodetection. Sensors. 9: 2524-2537. 10. Blanchette, C.D., Segelke, B.W., Fischer, N., Corzett, M., Kuhn, E.A., Cappuccio, J.A., Benner, H., Coleman, M.A., Chromy, B.A., Bench, G., Hoeprich, P.D. and Sulchek, T.A. (2009) Characterization and purification of polydispere reconstituted lipoproteins and nanolipoprotein particles. Int J Mol Sci. 10:2958-71. 11. Baker, S.E., Hopkins, R.C., Blanchette, C.D., Walsworth, V.L., Sumbad, R., Fischer, N.O., Kuhn, E.A., Coleman, M.A., Chromy, B.A., Létant, S.E., Hoeprich, P.D., Adams, M.W., and Henderson, P.T. (2009) Active Membrane-Bound Hydrogenase Incorporation into Soluble Nanoparticles. J. Am. Chem. Soc. 22:7508-9. 12. Cappuccio, J.A. Hinz, A.K., Kuhn, E.A. Fletcher, J., Arroyo, E.S., Henderson, P.T., Blanchette, C.D., Walsowrth, V.L., Corzett, M.H., Law, R.J., Pesavento, J.B., Segelke, B.W., Sulchek, T.A., Chromy, B.A., Katzen, F., Peterson, T., Bench, G., Kudlicki, W., Hoeprich. Craig D., and Coleman, M.A. High Throughput Protein Expression and Purification, Methods in Molecular Biology , Vol. 498, Doyle, Sharon A. (Ed.), 2009, chapt. 18, Cell-free Expression for Nanolipoprotein Particles (NLPs): Building a High-Throughput Membrane Protein Solubility Platform. 13. Peterson, L.E., Coleman, M.A. (In press) Logistic Ensembles of Random Spherical Linear Oracles for Microarray Classification. Int. J. Data Mining and Bioinformatics. 14. Martínez, A., Blanco, B., Carnevale, A., Mora, M.A., Coleman, M.A. Romero-Talamás, C.A. and Frías, S. (In Press) Assessment of immediate DNA damage to workers occupationally exposed to low dose of ionizing radiation using the Comet Assay. Professional affiliations: American Chemical Society Radiation Research Society Protein Society Environmental Mutagen Society Contact: Matthew A. Coleman Ph.D. Associate Adjunct Professor Radiation Oncology, Cancer Center University of California 4501 X Street, Suite G-140 Sacramento, CA 95817 tel: 916-734-5022 matthew.coleman@ucdmc.ucdavis.edu Senior Biomedical Scientist Biology and Biotechnology Division Lawrence Livermore National Laboratory Livermore, California, 94550 tel: (925)-423-7687 email: coleman16@llnl.gov |
Research > Meet the Researcher >